Acute toxicity study of bioactive galactomannans from seeds of two non-traditional leguminosae

Fabrícia da Cunha Jácome Marques; Francisco Glerison da Silva Nascimento; Dayanne Terra Tenório Nonato; Ana Maria Sampaio Assreuy; Edna Maria Camelo Chaves; Gislei Frota Aragão; Pedro Marcos Gomes Soares; Rondinelle Ribeiro Castro

doi:10.1590/1983-21252025v3812704rc

Autores

Fabrícia da Cunha Jácome Marques Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-1560-0741
Francisco Glerison da Silva Nascimento Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0003-2481-5733
Dayanne Terra Tenório Nonato Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil https://orcid.org/0000-0001-9477-1866
Ana Maria Sampaio Assreuy Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-2323-5385
Edna Maria Camelo Chaves Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0001-9658-0377
Gislei Frota Aragão Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-0689-8371
Pedro Marcos Gomes Soares Department of Morphology, Universidade Federal do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0003-0606-2539
Rondinelle Ribeiro Castro Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-9399-4109

DOI:

https://doi.org/10.1590/1983-21252025v3812704rc

Palavras-chave:

Polissacarídeo. Galactomanana. Segurança. Flor-de-pavão. Flamboiã.

Resumo

Fontes não tradicionais de galactomananas são amplamente cultivadas na Região Nordeste do Brasil, como as sementes das espécies ornamentais Caesalpinia pulcherrima (flor-de-pavão) e Delonix regia (flamboiã). As galactomananas GM-CP e GM-DR, extraídas respectivamente dessas sementes, estão sendo estudadas como potenciais agentes terapêuticos, mas avaliações sistemáticas sobre a toxicidade aguda das mesmas ainda não foram relatadas. Grupos de três ratas receberam GM-CP ou GM-DR oral (300 mg kg^-1), enquanto um grupo controle recebeu veículo (solução salina). Como não houve letalidade, outros grupos receberam doses de 2000 mg kg^-1, o que também não causou letalidade. A coleta de órgãos e amostras de sangue foi feita no dia 14. A hipernocicepção mecânica e o influxo de células inflamatórias foram medidos em outros grupos que receberam doses intra-articulares de GM-CP ou GM-DR (200 µg, n = 5 por grupo). Ambas as galactomananas não evocaram alterações fisiológicas/comportamentais ou inflamação articular. Como a DL50 não foi inferior a 2000 mg kg^-1, tais polissacarídeos podem ser alocados na classe 5 do Sistema Globalmente Harmonizado para Classificação e Rotulagem de Produtos Químicos.

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