Acute toxicity study of bioactive galactomannans from seeds of two non-traditional leguminosae

Fabrícia da Cunha Jácome Marques; Francisco Glerison da Silva Nascimento; Dayanne Terra Tenório Nonato; Ana Maria Sampaio Assreuy; Edna Maria Camelo Chaves; Gislei Frota Aragão; Pedro Marcos Gomes Soares; Rondinelle Ribeiro Castro

doi:10.1590/1983-21252025v3812704rc

Authors

Fabrícia da Cunha Jácome Marques Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-1560-0741
Francisco Glerison da Silva Nascimento Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0003-2481-5733
Dayanne Terra Tenório Nonato Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil https://orcid.org/0000-0001-9477-1866
Ana Maria Sampaio Assreuy Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-2323-5385
Edna Maria Camelo Chaves Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0001-9658-0377
Gislei Frota Aragão Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-0689-8371
Pedro Marcos Gomes Soares Department of Morphology, Universidade Federal do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0003-0606-2539
Rondinelle Ribeiro Castro Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-9399-4109

DOI:

https://doi.org/10.1590/1983-21252025v3812704rc

Keywords:

Polysaccharide. Galactomannan. Safety. Peacock flower. Flamboyant.

Abstract

Non-traditional galactomannans sources are widely cultivated in the Brazilian Northeast Region, such as the species Caesalpinia pulcherrima (peacock flower) and Delonix regia (flamboyant). The galactomannans GM-CP and GM-DR were extracted from the respective seeds and are being studied as potential therapeutic agents, but systematic evaluations on their acute toxicity are yet to be reported. Groups of three female rats received oral GM-CP or GM-DR (300 mg kg^-1), whereas a control group received vehicle (saline). Since there was no lethality, other groups received doses of 2000 mg kg^-1, which also did not cause lethality. Organs and blood samples were collected on day 14. Mechanical hypernociception and inflammatory cell influx were measured in other groups receiving intra-articular doses of GM-CP or GM-DR (200 µg, n = 5 per group). Neither galactomannan evoked physiological / behavioral changes or joint inflammation. Since the LD50 was less than 2000 mg kg^-1, such polysaccharides may be allocated in the class 5 of the Globally Harmonized System for Classification and Labelling of Chemicals.

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