Saccharomyces cerevisiae strain associated to a fish culture additive: antifungal activity and AFB1 adsorption potential

Abstract

Saccharomyces cerevisae is widely applied as a probiotic in aquaculture activities, due to its ability to decontaminate the mycotoxin Aflatoxin B₁ (AFB₁). With this in mind, the aim of the present study was to evaluate the antifungal and anti-aflatoxigenic activities of inactivated Saccharomyces cerevisiae mixed with a commercial product (CP) of animal feed to assess its influence on the Aspergillus flavus and A. parasiticus fungi growth and on the AFB₁ production. In addition, AFB₁ adsorption potential of S. cerevisae and commercial product was also investigated. Different concentrations of commercial product alone and in the presence of inactivated yeast were analyzed by Aspergillus growth inhibition test, aflatoxin production by Aspergillus species and AFB₁ adsorption capacity. AFB₁ detection and quantification were carried out by High Performance Liquid Chromatography. The inactivated yeast and commercial product combination was effective in reducing A. flavus and A. parasiticus growth. A. flavus produced less AFB₁ after the inactivated yeast treatment, whereas A. parasiticus produced significantly less AFB₁ under a combination of inactivated yeast and 50% CP. Regarding AFB₁ adsorption, 100% CP displayed the highest adsorption capacity at 10 ng mL^-1 AFB₁. At 25 ng mL^-1 AFB₁, only the treatment comprising inactivated yeast associated to 50% CP led to AFB₁ adsorption, albeit at low levels.