PHYSIOLOGICAL CHARACTERIZATION OF DIAZOTROPHIC BACTERIA ISOLATED FROM Brachiaria brizantha RHIZOSPHERE

Authors

  • Ana Beatriz Carvalho Terra Department of agricultural science, Universidade José do Rosário Vellano, Alfenas, MG http://orcid.org/0000-0002-1912-0059
  • Flávia Romam da Costa Souza Department of agricultural science, Universidade José do Rosário Vellano, Alfenas, MG http://orcid.org/0000-0003-0482-822X
  • José Ricardo Mantovani Department of agricultural science, Universidade José do Rosário Vellano, Alfenas, MG http://orcid.org/0000-0002-9817-8143
  • Adauton Vilela Rezende Department of agricultural science, Universidade José do Rosário Vellano, Alfenas, MG http://orcid.org/0000-0001-8515-484X
  • Ligiane Aparecida Florentino Department of agricultural science, Universidade José do Rosário Vellano, Alfenas, MG http://orcid.org/0000-0001-9092-3017

DOI:

https://doi.org/10.1590/1983-21252019v32n310rc

Keywords:

Plant growth-promoting bacteria. Soil microorganisms. Sustainability in animal production.

Abstract

The objective of this study was to evaluate the ability of diazotrophic bacteria isolated from Brachiaria brizantha rhizosphere to grow at different pH values and salt concentrations, to produce indoleacetic acid (IAA), and to solubilize phosphate. Both acidity and salinity tolerance tests were performed on modified solid FAM medium. Acidity resistance tests were performed at pH values of 4.0, 5.0, 6.0, and 7.0, while, salt resistance was evaluated at concentrations of 0.01, 5.02, 9.99, 14.96, 19.98, 24.95, 29.98, 34.94, 39.97, 44.94, and 49.96 gL-1 NaCl. For the IAA production assay, bacterial strains were cultivated in liquid DYGS medium both in the absence and presence of tryptophan (Trp). In addition, phosphorus solubilization assay was performed in GL liquid medium. All strains grew at every pH value tested, and a high diversity was observed after salt resistance, IAA production, and phosphate solubilization testing. Strains UNIFENAS 100-51, UNIFENAS 100-52, UNIFENAS 100-60, UNIFENAS 100-63, and UNIFENAS 100-65 were those with the best growth at the highest salt concentrations. Furthermore, in the presence of Trp, strains UNIFENAS 100-63 and UNIFENAS 100-69 were the ones with the highest IAA production. Strain UNIFENAS 100-52 showed the best response to the in vitro phosphate solubilization assay. Based on these results, it can be seen that studies related to the physiological and metabolic characteristics of diazotrophic bacterial strains are important to ensure greater success in the field.

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Published

26-08-2019

Issue

Section

Agronomy