Growth and solute accumulation in collard greens after pre-treatment with H2O2 under salt stress
DOI:
https://doi.org/10.1590/1983-21252025v3812861rcKeywords:
Brassica oleracea. Salinity. ROS. Biomass production.Abstract
Hydrogen peroxide (H2O2) acts as a signaling molecule inducing increased plant tolerance to stress conditions. The objective of the present study was to evaluate the effect of pre-treatment with H2O2 as a possible attenuator of salt stress on the production of biomass and organic and inorganic solutes in collard greens under salt stress. The plants were grown under hydroponic conditions in a greenhouse. The experimental design used was completely randomized with eight replications with the combination of three times of exposure to H2O2 (12, 24, and 36 h) and four levels of H2O2 in the pre-treatment solution (0.1, 1.0, 10, and 100 µM). Two control treatments were added, one without the presence of NaCl and another with the presence of 100 mM NaCl, both without pre-treatment with H2O2. After 60 days of assay the plants were collected and subsequently analyzed. Salinity affected collard green biomass production regardless of the concentration and time of exposure to H2O2. Salinity reduced biomass production in collard greens and there was an increase in Na+ and Cl-, while K+ decreased, but pre-treatment with H2O2 proved to be effective in increasing the levels of organic solutes, highlighting the importance of H2O2 in the plant's adaptive response.
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