Powdery mildew resistance in pumpkin sub-accessions from the vegetable germplasm bank

Francisco Davi da Silva; Patrícia Moreira Valente; Maria da Cruz Chaves Lima Moura; Manoel Abílio de Queiroz; Fernando Antonio Souza de Aragão; Carlos Nick Gomes

doi:10.1590/1983-21252025v3812701rc

Authors

Francisco Davi da Silva Natural History Museum and Botanical Garden, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil https://orcid.org/0000-0001-8679-3539
Patrícia Moreira Valente Department of General Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil https://orcid.org/0000-0002-5840-9103
Maria da Cruz Chaves Lima Moura Universidade Federal do Maranhão, São Luiz, MA, Brazil https://orcid.org/0000-0003-1282-5328
Manoel Abílio de Queiroz Department of Technology and Social Sciences, Universidade do Estado da Bahia, Campus Juazeiro, BA, Brazil https://orcid.org/0000-0001-9501-2343
Fernando Antonio Souza de Aragão Embrapa Agroindústria Tropical, Fortaleza, CE, Brazil https://orcid.org/0000-0002-4041-7375
Carlos Nick Gomes Department of Agronomy, Universidade Federal de Viçosa, Viçosa, MG, Brazil https://orcid.org/0000-0001-5990-255X

DOI:

https://doi.org/10.1590/1983-21252025v3812701rc

Keywords:

Cucurbit. Pre-breeding. Genetic resources. Plant resistance.

Abstract

Powdery mildew is one of the most challenging diseases for pumpkin and squash production, as it can cause significant losses in fruit yield and quality. The objective of this study was to evaluate sub-accessions of Cucurbita moschata from the Vegetable Germplasm Bank of the Federal University of Viçosa (BGH-UFV), in Viçosa, Minas Gerais, Brazil, for resistance to powdery mildew (Podosphaera xanthii) in young and adult plants. The physiological race of P. xanthii used in the experiments was identified using nine differentials. Eighty-seven S₀ sub-accessions were evaluated, along with two commercial cultivars of C. moschata as controls. A completely randomized experimental design with three replications was used to evaluate young plants, whereas Federer's augmented block design was used to evaluate adult plants, with seven replications as controls. Inoculation consisted of depositing spores and allowing contact between diseased and healthy plants. Evaluations were based on a subjective rating scale, with estimation of the relative area under the disease progress curve. The P. xanthii used in the experiments was identified as race 2US, making this study the first report of this variant in Minas Gerais. A significant difference was found among the C. moschata sub-accessions regarding their response to powdery mildew, indicating the existence of genetic variability and allowing for the selection of genotypes. Fifteen sub-accessions of the evaluated germplasm were identified as promising sources for developing lines resistant to powdery mildew.

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