Biocontrol of Pratylenchus zeae and sugarcane growth promotion by rhizobacteria

Cielo Pamela Machaca Calsin; Ismail Teodoro  Souza Junior; Jaqueline Tavares Schafer; Matheus Martins Pereira; Sabrina Oliveira Martins; Andrea Bittencourt Moura Baccarin; César Bauer Gomes

doi:10.1590/1983-21252025v3812616rc

Authors

Cielo Pamela Machaca Calsin Crop Protection Graduate Program, Faem, Universidade Federal de Pelotas, Pelotas, RS, Brazil https://orcid.org/0000-0003-1899-3883
Ismail Teodoro Souza Junior Centro Universitário de Várzea Grande, Cuiabá, MT, Brazil https://orcid.org/0000-0002-0023-7329
Jaqueline Tavares Schafer AgroBianchini, Santo Ângelo, RS, Brazil https://orcid.org/0000-0002-8411-3357
Matheus Martins Pereira Crop Protection Graduate Program, Faem, Universidade Federal de Pelotas, Pelotas, RS, Brazil https://orcid.org/0009-0009-8717-2166
Sabrina Oliveira Martins Universidade Federal de Pelotas, Pelotas, RS, Brazil https://orcid.org/0000-0003-0992-865X
Andrea Bittencourt Moura Baccarin Plant Protection Department, Faem, Universidade Federal de Pelotas, Pelotas, RS, Brazil https://orcid.org/0000-0002-6781-4231
César Bauer Gomes Embrapa Clima Temperado, Pelotas, RS, Brazil https://orcid.org/0000-0002-3247-728X

DOI:

https://doi.org/10.1590/1983-21252025v3812616rc

Keywords:

Biological control, PGPR, Root-lesion nematode, Saccharum spp.

Abstract

The root lesion nematode (Pratylenchus zeae) is one of the main phytosanitary problems of sugarcane, and the biological control is an important tool in the integrated nematode management. Thus, the objective of this study was to evaluate the potential of rhizobacteria in the biocontrol of P. zeae and their plant growth promotion (PGPR) in sugarcane. Seedlings of sugarcane ‘RB008347’ were microbiolized with individual suspensions of nine bacterial isolates in a greenhouse. After 15 days, the plants were transplanted to pots containing sterilized soil, and then were inoculated or not with 1000 specimens of P. zeae/plant under greenhouse conditions. Non-microbiolized seedlings inoculated or not with the nematode were used as controls. Ninety days after inoculation, each plant was evaluated for its development, number of nematodes/root system, nematode/g of roots and nematode reproduction factor. In an in vitro bioassay, the nematicidal activity of these bacteria on specimens of P. zeae was evaluated. Additionally, the bacterial isolates were characterized biochemically regarding production of compounds related to nematode biocontrol and PGPR. The majority of isolates promoted significant increases in the number of tillers, shoot fresh and root fresh mass, and suppressed P. zeae reproduction (48-74%) compared to the control. In the in vitro bioassays, the isolates XT23, XT26, XT51, XT56, XT37 and P17 showed nematicidal activity and produced at least one compound related to the biological control of phytonematodes and PGPR. In this way, the microbiolization of sugarcane seedlings with rhizobacteria demonstrates its effectiveness as a bionematicide and growth promoter for sugarcane crops.

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Author Biography

César Bauer Gomes, Embrapa Clima Temperado, Pelotas, RS, Brazil

Agrônomo, Dr. Nematologista

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