Virulência de fungos entomopatogênicos sobre larvas de Lepidoptera: Noctuidae

Elisângela de Souza Loureiro; Pamella  Mingotti Dias; Luis Gustavo Amorim Pessoa; Tatiana Souza do  Amaral; Gabriella Silva de Gregori; Andressa Silva Rodrigues; Maurício Sekiguchi de Godoy

doi:10.1590/1983-21252024v3712375rc

Authors

Elisângela de Souza Loureiro Universidade Federal de Mato Grosso do Sul, Chapadão do Sul, MS, Brazil https://orcid.org/0000-0002-9708-3775
Pamella Mingotti Dias Faculty of Biological and Environmental Sciences, Universidade Federal da Grande Dourados, Dourados, MS, Brazil https://orcid.org/0000-0002-0963-9455
Luis Gustavo Amorim Pessoa Universidade Federal de Mato Grosso do Sul, Chapadão do Sul, MS, Brazil https://orcid.org/0000-0003-4646-062X
Tatiana Souza do Amaral Universidade Federal de Mato Grosso do Sul, Chapadão do Sul, MS, Brazil https://orcid.org/0000-0001-5674-4998
Universidade Federal de Mato Grosso do Sul, Chapadão do Sul, MS, Brazil https://orcid.org/0000-0002-2601-8511
Gabriella Silva de Gregori Universidade Federal de Mato Grosso do Sul, Chapadão do Sul, MS, Brazil https://orcid.org/0009-0003-8577-0986
Andressa Silva Rodrigues Universidade Federal de Mato Grosso do Sul, Chapadão do Sul, MS, Brazil https://orcid.org/0000-0001-5602-1474
Maurício Sekiguchi de Godoy Plant Health Department, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil https://orcid.org/0000-0002-3842-340X

DOI:

https://doi.org/10.1590/1983-21252024v3712375rc

Keywords:

Biological control. Beauveria. Metarhizium. Helicoverpa. Pest management.

Abstract

Currently, agricultural cropping systems have adopted integrated pest management (IPM) as a successful model for pest control. The use of entomopathogenic fungi in IPM has increased because of their great potential for reducing arthropod pest populations without causing harm to human health and ecosystems. Beauveria bassiana and Metarhizium anisopliae stand out among the most used fungi in biological control, with many isolates commercialized worldwide. Helicoverpa armigera is an agriculturally important pest in Brazil and worldwide, causing damage to several crops. In this context, the objective of this study was to evaluate the biological control potential of Beauveria bassiana, Metarhizium anisopliae, and M. rileyi on H. armigera. The results indicated that the entomopathogenic fungi B. bassiana (strain ESALQ PL63), M. anisopliae (strain ESALQ E9), and M. rileyi (strain UFMS 03), applied using different methods (direct application and dry film) and concentrations (1 × 10⁷, 1 × 10⁸, and 1 × 10⁹ conidia mL^-1), resulted in low mortality and no virulence in first-, third-, and fifth-instar larvae of H. armigera. The death of all individuals subjected to treatment with B. bassiana (strain ESALQ PL63) was confirmed by conidiogenesis. Considering the importance of H. armigera as an agricultural pest and the biodiversity of entomopathogenic fungi in Brazil, further investigations on the virulence of fungal strains are necessary to improve the integrated management of lepidopteran pests through microbial control, explore the potential of new strains, and understand the relationships between microorganisms and host defense mechanisms.

Downloads

Download data is not yet available.

Author Biography

Elisângela de Souza Loureiro, Universidade Federal de Mato Grosso do Sul, Chapadão do Sul, MS, Brazil

References

AGBOYI, L. K. et al. Improving the efficiency of Beauveria bassiana applications for sustainable management of Plutella xylostella (Lepidoptera: Plutellidae) in West Africa. Biological Control, 144: 1-9, 2020.

BORGES, F. S. P. et al. Performance of phytosanitary products for control of soybean caterpillar. Annals of the Brazilian Academy of Sciences, 93: 1-10, 2021.

COSTA, V. H. D. et al. Nomuraea rileyi (Hypocreales: Clavicipitaceae) in Helicoverpa armigera (Lepidoptera: Noctuidae) larvae in Brazil. Florida Entomologist, 980: 796-798, 2015.

CRUZ-AVALOS, A. M. et al. High Virulence of Mexican Entomopathogenic Fungi Against Fall Armyworm, (Lepidoptera: Noctuidae). Journal of Economic Entomology, 112: 99-107, 2019.

CZEPAK, C. et al. First record of occurrence of Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil. Pesquisa Agropecuária Tropical, 43: 110-113, 2013.

DIAS, P. M. et al. Interactions between Fungal-Infected Helicoverpa armigera and the Predator Chrysoperla externa. Insects, 10: 1-11, 2019.

DIAS, P. M. et al. Selectivity of Entomopathogenic Fungi to Chrysoperla externa (Neuroptera: Chrysopidae). Insects, 11: 1-14, 2020.

FARIA, M. et al. What would be representative temperatures for shelf-life studies with biopesticides in tropical countries? Estimates through long-term storage of biocontrol fungi and calculation of mean kinetic temperatures. BioControl, 67: 2213-224, 2022.

FATHIPOUR, Y.; SEDARATIAN, A. Integrated Management of Helicoverpa armigera in Soybean Cropping Systems. In: EL-SHEMY, H. (Ed.), Soybean-Pest Resistance. Rijeka, Croatia: InTech, 2013. v. 1, cap. 9, p. 231-280.

FITE, T. et al. Evaluation of Beauveria bassiana, Metarhizium anisopliae, and Bacillus thuringiensis for the management of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) under laboratory and field conditions. Biocontrol Science and Technology, 30: 1-18, 2019.

GASMI, L. et al. Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana. Scientific Reports, 11: 91-103, 2021.

GOTTI, I. A. et al. Blastospores from Metarhizium anisopliae and Metarhizium rileyi Are Not Always as Virulent as Conidia Are towards Spodoptera frugiperda Caterpillars and Use Different Infection Mechanisms. Microorganisms, 11: 1-16, 2023.

GREENE, G. L.; LEPPLA, N. C.; DICKERSON, W. A. Velvet bean caterpillar: The rearing procedure artificial medium. Journal Economic Entomology, 69: 487-488, 1976.

HASSAN, S. A. Standard methods a test the Sideeffects of pesticides on natural enemies of insects and mites developed by the IOBC/WPRS Working Group ‘Pesticides and Benefitial Organisms”. Bulletin OEPP/EPPO, 15: 214-255, 1985.

IDREES, A. et al. Effectiveness of Entomopathogenic Fungi on Immature Stages and Feeding Performance of Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae. Insects, 12: 1044-1056, 2021.

JAFARPOUR, M. et al. Functional significance of graded properties of insect cuticle supported by an evolutionary analysis. Interface, 17: 1-8, 2020.

KALVNADI, E. et al. Sublethal concentrations of the entomopathogenic fungus, Beauveria bassiana, increase the costs of physical conditioning of pups of Helicoverpa armigera (Lepidoptera: Noctuidae). Journal Invertebrate Pathology, 158: 32-42, 2018.

KUMAR, A.; SHARFUDDIN, C. Evaluation of Beauveria bassiana and Metarhizium anisopliae, for the management of Helicoverpa armigera (Hubner). Journal Mycopathological Research, 60: 91-97, 2022.

LOPES, R. B. et al. Biological Control in Latin America. Neotropical Entomology, 52: 119-121, 2023.

LOUREIRO, E. S. et al. Hydration levels on conidial production of Metarhizium rileyi (Ascomycota) in solid growing medium. Revista de Agricultura Neotropical, 6: 19-23, 2019.

MONTECALVO, M. P.; NAVASERO, M. M. Comparative virulence of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metchnikoff) Sorokin to Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). Journal of the International Society for Southeast Asian Agricultural Sciences, 27: 15-26, 2021.

PARRA, J. R. P. Biological Control in Brazil: state of art and perspectives. Scientia Agricola, 80: 1-4, 2023.

RAMANUJAM, B.; POORNESHA, B.; SHYLESHA, A. N. Effect of entomopathogenic fungi against invasive pest Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in maize, Egyptian Journal Biological Pest Control, 30: 1-5, 2020.

SEYEDTALEBI, F. S. et al. Quantitative comparison for some immune responses among Eurygaster integriceps, Ephestia kuehniella and Zophobas morio against the entomopathogenic fungus Beauveria bassiana. Invertebrate Survival Journal, 14: 174-181, 2017.

SHEHZAD, M. et al. On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). Egyptian Journal Biological Pest Control, 31: 1-7, 2021.

TANG, G.; HOU, R. Effects of environmental factors on the virulence of the entomopathogenic fungus Nomuraea rileyi, against the ear of corn earworm, Helicoverpa armigera (Lep. Noctuidae). Journal Applied Entomology, 125: 243-248, 2001.

VIANNA, M. F. et al. ISSR markers to explore entomopathogenic fungi genetic diversity: Implications for biological control of tobacco pests. Journal of Biosciences, 45: 1-11, 2020.

WANG, C.; FENG, M. G. Advances in fundamental and applied studies in China of fungal biocontrol agents for use against arthropod pests. Biological Control, 68: 129-135, 2014.