Prospecting plant growth-promoting rhizobacteria in grapevines in the São Francisco Valley

José Petrônio  Mendes Júnior; Giselle Gomes Monteiro  Fracetto; Felipe José Cury Fracetto; Davi José Silva; Mario de Andrade Lira Junior; Felipe Martins do Rêgo Barros

doi:10.1590/1983-21252024v3711523rc

Authors

José Petrônio Mendes Júnior Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0002-7076-532X
Giselle Gomes Monteiro Fracetto Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0001-9366-2588
Felipe José Cury Fracetto Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0002-9112-1339
Davi José Silva Embrapa Semiárido, Petrolina, PE, Brazil https://orcid.org/0000-0003-0447-2999
Mario de Andrade Lira Junior Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0001-7779-3369
Felipe Martins do Rêgo Barros Department of Soil Science, Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Piracicaba, SP, Brazil https://orcid.org/0000-0002-5795-9402

DOI:

https://doi.org/10.1590/1983-21252024v3711523rc

Keywords:

Viticulture. Semiarid. Auxin. Phosphate solubilization. Antibiosis.

Abstract

Viticulture is the main agricultural production in the São Francisco Valley, Brazil; however, farm soil management systems require large volumes of fertilizer that could contribute to climate change. Therefore, using plant growth-promoting rhizobacteria (PGPR) has been reported to reduce or replace plant input. This study aimed to evaluate the bacteria in the rhizosphere of Vitis vinifera cultivated in the São Francisco Valley in Pernambuco, Brazil, and to characterize the mechanisms that promote plant growth. The PGPR with the greatest biotechnological potential was identified using sequencing. The bacteria were isolated from tryptic soy agar (TSA) culture medium inoculated with 100 µL of a serial dilution. The isolates obtained were characterized phenotypically and tested for their ability to solubilize phosphate, promote biological nitrogen fixation (BNF), and produce indole-3 acetic acid (IAA), biofilms, and antibiotic factors against Xanthomonas campestris pv. viticola. A total of 423 bacteria were obtained, of which 99 presented positive results for at least one of the growth-promotion mechanisms, representing 6.85% for phosphate solubilizers, 0.74% for BNF, 5.7% for IAA synthesizers, 11.27% for biofilm producers, and 4.01% for promoting antibiosis against X. campestris pv. viticola. Isolates 3.19 and 31.14; 3.17 and 17.04; 5.35 and 5.42; and 5.37 identified as Stenotrophomonas, Bacillus, Pseudomonas, and Clostridium, respectively, presented a biotechnological potential for future experiments to promote vine growth.

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