Prospecting plant growth-promoting rhizobacteria in grapevines in the São Francisco Valley

José Petrônio Mendes Júnior; Giselle Gomes Monteiro  Fracetto; Felipe José Cury Fracetto; Davi José Silva; Mario de Andrade Lira Junior; Felipe Martins do Rêgo Barros

doi:10.1590/1983-21252024v3711523rc

Autores

José Petrônio Mendes Júnior Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0002-7076-532X
Giselle Gomes Monteiro Fracetto Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0001-9366-2588
Felipe José Cury Fracetto Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0002-9112-1339
Davi José Silva Embrapa Semiárido, Petrolina, PE, Brazil https://orcid.org/0000-0003-0447-2999
Mario de Andrade Lira Junior Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil https://orcid.org/0000-0001-7779-3369
Felipe Martins do Rêgo Barros Department of Soil Science, Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Piracicaba, SP, Brazil https://orcid.org/0000-0002-5795-9402

DOI:

https://doi.org/10.1590/1983-21252024v3711523rc

Palavras-chave:

Viticultura. Semiárido. Auxinas. Solubilização de fosfato. Antibiose.

Resumo

A viticultura é a principal produção agrícola do Vale do São Francisco, Brasil; no entanto, os sistemas agrícolas de manejo dos solos requerem grandes volumes de fertilizantes que podem contribuir para as alterações climáticas. Portanto, foi relatado que o uso de rizobactérias promotoras de crescimento de plantas (RPCP) reduz ou substitui insumos vegetais. Este estudo teve como objetivo avaliar as bactérias da rizosfera de Vitis vinifera cultivada no Vale do São Francisco, em Pernambuco, Brasil, e caracterizar os mecanismos que promovem o crescimento das plantas. As RPCP com maior potencial biotecnológico foram identificadas por meio de sequenciamento. As bactérias foram isoladas em meio de cultura Agar Triptona de Soja (TSA) inoculado com 100 µL de uma diluição seriada. Os isolados obtidos foram caracterizados fenotipicamente e testados quanto à capacidade de solubilizar fosfato, promover fixação biológica de nitrogênio (FBN) e produzir ácido indol-3 acético (AIA), biofilmes e fatores antibióticos contra Xanthomonas campestris pv. vitícola. Um total de 423 bactérias foi obtido, das quais 99 apresentaram resultados positivos para pelo menos um dos mecanismos de promoção de crescimento, representando 6,85% para solubilizadores de fosfato, 0,74% para FBN, 5,7% para sintetizadores de AIA, 11,27% para produtores de biofilme e 4,01% para promoção de antibiose contra X. campestris pv. vitícola. Os isolados 3.19 e 31.14; 3.17 e 17.04; 5.35 e 5.42; e 5.37 identificados como Stenotrophomonas, Bacillus, Pseudomonas e Clostridium, respectivamente, apresentaram potencial biotecnológico para futuros experimentos para promover o crescimento de videiras.

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