Morphometric evaluation of the spermatogenic process of adults wistar rats exposed to the 2,4diclorophenoxiacetic acid associated to picloram (TORDON 2,4-D® 64/240 BR)

Abstract

Thirty adult male wistar rats were used in five groups. Two groups received oral treatment with aqueous solution of TORDON 2,4-D® 64/240 BR in different concentrations; group O1: 2 ml.L-1 (n = 6); group O2: 4 ml.L-1 (n = 6). Two groups received topical treatment in the dorsal cervical area, the following concentrations were used, group T1: 16.7 ml.L-1 (n = 6), and group T2: 400 ml.L-1 (n = 6). A control group was used without the addition of the herbicide (n = 6). At the end of the experiment, the animals were euthanized; the testes were collected and fixed for subsequent histological analysis in light microscope. The T1 group showed increase in albuginea weight (P < 0.05). The animals of the O2 group showed significant reduction (P < 0.05) of the height of seminiferous epithelium. The animals of the O1 and T2 groups did not present harmful effects on the spermatogenic morphometry.