In vitro effect of polar extracts from Annona glabra seeds on Fusarium solani

Oscar Marino Mosquera Martínez; Ana Isabel Giraldo Rivera; Gloria Edith Guerrero Álvarez; Norma Patricia Durán Osorio; Nathalia Cardona Hurtado

doi:10.1590/1983-21252024v3711810rc

Autores

Oscar Marino Mosquera Martínez Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0003-2998-0842
Ana Isabel Giraldo Rivera Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-4733-5460
Gloria Edith Guerrero Álvarez Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-0529-5835
Norma Patricia Durán Osorio Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-2711-6842
Nathalia Cardona Hurtado Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-3814-4726

DOI:

https://doi.org/10.1590/1983-21252024v3711810rc

Palavras-chave:

Annonaceae. Acetogeninas. Alcaloides. Controle biológico. Terpenoides.

Resumo

O gênero Fusarium é causador de doenças em diversas culturas agrícolas em todo o mundo com perdas econômicas significativas. Os fungicidas químicos são a principal estratégia de controle, mas o seu uso intensivo e prolongado gera resistência, resíduos tóxicos e contaminação ambiental. O controle biológico a partir de substâncias bioativas tem ampla aplicação. Neste estudo, a atividade fungicida do extrato obtido de Annona glabra L. como alternativa ao controle de Fusarium solani. Além disso, foi realizada uma caracterização química com o intuito de identificar os grupos de metabólitos presentes no extrato. A atividade fungicida contra F. solani foi avaliada pelo método de microdiluição e foi estabelecida a concentração inibitória mínima (CIM) e a concentração inibitória média (IC₅₀). Os resultados da atividade fungicida mostraram que o IC₅₀ do extrato foi de 972.24 mg L⁻¹ e o MIC foi de 12.02 mg L⁻¹. Através da caracterização química por cromatografia líquida de ultra-alta eficiência acoplada à espectrometria de massas (UHPLC-MS/MS), foi confirmada a presença de acetogênios THF, alcaloides isoquinolínicos e diterpenos do tipo caureno, que são os principais responsáveis pela atividade avaliada. Os resultados obtidos fornecem evidências de que os extratos de sementes de A. glabra podem ser uma fonte potencial de metabólitos com atividade fungicida contra F. solani.

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